Effects of P. aeruginosa on A. fumigatus conidia (A) and sporelings (B) in cocultures.A. fumigatus conidia (A) and sporelings (B) at a density of 1 × 106 cells/ml were incubated with P. aeruginosa cells ranging from 1 x 101-1 x 106 cells/ml in 1 ml SD broth at 35°C for 24 h. At the end of the incubation the adherent microbial growth containing fungal and bacterial cells were washed 3 times with distilled water (1 ml each) and the viability of the cells was determined by CFU assay. In all mixed cultures the P. aeruginosa CFUs were similar (≈1 × 1010 CFU/ml). The experiment was performed at two different times with AF53470 and PA56402 using independently prepared conidial suspensions and bacterial cultures, and one time with AF36607 and PA27853. Similar results were obtained for the clinical and the laboratory isolates. The vertical bar on each data point represents the standard error of the mean for two independent experiments with AF53470 and PA56402. The data were analyzed by one way ANOVA with Dunnett multiple comparison test where the control was compared with each of the experimental group using GraphPad Prism 5.0.
Manavathu et al. BMC Microbiology 2014 14:53 doi:10.1186/1471-2180-14-53