Table 3

Effects of homologous sequence length on CAR2 deletion frequency
Homology length (bp) Gene deletion frequencya Improvement (folds)
WT ∆ku70e
50 0 (780) 0 (8) -
100 0 (620) 21.4% (14) -
250 0.7% (1668) 30.3% (33) 43.3
500 11.2% (2124) 67.0% (778) 6
750 10.5% (6152) 75.3% (885) 7.2
1000 30.4% (2280) 91.7% (2196) 3
1500 20.5% (2730) 91.0% (4304) 4.4

Note: aNumber in parenthesis indicate number of transformants screened.

Koh et al.

Koh et al. BMC Microbiology 2014 14:50   doi:10.1186/1471-2180-14-50

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