Targeted deletion of STE20 and URA3 genes. (A) and (D) Illustration of gene deletion constructs; (B) and (E): Southern blots using probes shown in (A) and (D); (C) Colony phenotype of WT and ∆ste20 strains mated with R. toruloides ATCC 10788; (F) Growth phenotype of WT and ∆ura3 strains derived from 10-fold serially diluted cells. The latter showed resistance to 5-FOA (1 g/L) – a substrate that can be converted to a toxic intermediate by the URA3-encoded enzyme .
Koh et al. BMC Microbiology 2014 14:50 doi:10.1186/1471-2180-14-50