Phenotypic and genotypic characterization of pKOCAR2 transformants. (A) A transformation plate showing both red and albino transformants, with black arrow heads marking some albino transformants. (B) Southern blot results using the 5′ flanking sequence of CAR2 as a probe. Genomic DNA was digested with PvuI and a band shift from 5.0 kb (WT) to 3.0 kb indicates successful deletion of CAR2 gene.
Koh et al. BMC Microbiology 2014 14:50 doi:10.1186/1471-2180-14-50