Table 2

Toxic activity ofP. syringaepv syringae UMAF0158 mutants andmgooperon complemented strains
Strains E. coliinhibition assay Mangotoxin production
PMS PMS + ornithine
Wild type strain and derivative mutants
UMAF0158 + - Yes
mboA- -* -* No
ΔmgoA - - No
gacA- - - No
gacS- - - No
Transformed with empty vector
UMAF0158 + - Yes
mboA- -* -* No
ΔmgoA - - No
gacA- - - No
gacS- - - No
Transformed with pLac-mgoBCAD
UMAF0158 ++ - Yes
mboA- -* -* No
ΔmgoA ++ - Yes
gacA- - - No
gacS- - - No

The results are indicated as follows: - absence of inhibition halo, + inhibition halo between 5-10 mm, ++ inhibition halo bigger 10 mm, -* slight toxicity which did not revert in presence of ornithine. Toxic activity, which reverts in the presence of ornithine, denotes the production of mangotoxin.

Carrión et al.

Carrión et al. BMC Microbiology 2014 14:46   doi:10.1186/1471-2180-14-46

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