Additional file 3: Figure S2.

Virulence analysis of the wild type strain P. syringae pv. syringae UMAF0158 and corresponding derivatives using a detached tomato leaf assay. (A) In planta growth inside the tomato leaflets after H2O2 surface disinfection of the wild type strain UMAF0158, mgoA and mboA mutants, and their respective complemented derivatives. (B) Severity of necrotic symptoms (necrotic area) on tomato leaflets inoculated with wild type strain UMAF0158, the mutants in mboA and mgoA with their respective complemented derivatives. The total necrotic area (mm2) from 30 inoculated points on tomato leaflets was measured 10 days after inoculation and used to compare the severity of necrotic symptoms produced by the different strains. (C) Representative pictures of the necrotic lesions produced by the wild type strain and the different mutants at 10 dpi. Different letters denote statistically significant differences at p = 0.05, according to analysis of variance followed by Fisher’s least significant difference test.

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Carrión et al. BMC Microbiology 2014 14:46   doi:10.1186/1471-2180-14-46