Neutralization assay for monkey sera using the new assay system. Samples #175 and #052 and #NS in various dilutions were mixed with 50 PFU Luc-DENV, after incubating for 24 h, mixtures were added to BHK-21 cells in 12-well plate. Luciferase activities were measured in lysed BHK-21 cells after 48 h incubating to assay neutralization activities. Error bars indicate the standard deviations from two independent experiments.
Song et al. BMC Microbiology 2014 14:44 doi:10.1186/1471-2180-14-44