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High prevalence and two dominant host-specific genotypes of Coxiella burnetii in U.S. milk

Talima Pearson1*, Heidie M Hornstra1, Remy Hilsabeck1, Lauren T Gates1, Sonora M Olivas1, Dawn M Birdsell1, Carina M Hall1, Sabrina German1, James M Cook1, Meagan L Seymour1, Rachael A Priestley2, Ashley V Kondas2, Christine L Clark Friedman1, Erin P Price14, James M Schupp3, Cindy M Liu13, Lance B Price3, Robert F Massung2, Gilbert J Kersh2 and Paul Keim13

Author Affiliations

1 Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ 86011-4073, USA

2 Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA

3 Pathogen Genomics Division, Translational Genomics Research Institute, Phoenix, AZ, USA

4 Current address: Global and Tropical Health Division, Menzies School of Health Research, Tiwi, Northern Territory 0810, Australia

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BMC Microbiology 2014, 14:41  doi:10.1186/1471-2180-14-41

Published: 17 February 2014

Additional files

Additional file 1:

Specific information pertaining to the treatment and storage of milk samples used in the study, DNA extraction methods used on the milk samples, and assay information including primer and probe sequences as well as PCR conditions. Also included are the methods for constructing self-reporting, synthetic positive control templates.

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