Open Access Research article

Identification of type III secretion substrates of Chlamydia trachomatis using Yersinia enterocolitica as a heterologous system

Maria da Cunha13, Catarina Milho14, Filipe Almeida13, Sara V Pais13, Vítor Borges2, Rui Maurício15, Maria José Borrego2, João Paulo Gomes2 and Luís Jaime Mota13*

Author Affiliations

1 Infection Biology Laboratory, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal

2 Department of Infectious Diseases, National Institute of Health, Lisbon, Portugal

3 Centro de Recursos Microbiológicos (CREM), Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal

4 Present address: Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, Portugal

5 Present address: Cambridge Institute for Medical Research, Addenbrooke’s Hospital, Hills Road, Cambridge, United Kingdom

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BMC Microbiology 2014, 14:40  doi:10.1186/1471-2180-14-40

Published: 17 February 2014

Additional files

Additional file 1: Table S1:

Plasmids used and constructed in this work.

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Additional file 2: Table S2:

Primers used in this work for construction of plasmids.

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Additional file 3: Table S3:

Summary of results obtained in analyses of T3S signals in proteins of Chlamydia trachomatis and comparison to in silico prediction methods.

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