The integrase-deficient lentivirus vector (IDLV) transfected U937 cells with high efficiency and the cells expressed Mtb Hsp16.3. An IDLV delivered the transgene into U937 macrophages for instantaneous expression. The fluorescence microscopy and flow cytometry were used at 64 h after infection to detect GFP and analyse the transduction efficiency. A, the transduction efficiency of the test group of U937 cells (expressing Mtb Hsp16.3 and GFP) was 73%. B, the transduction efficiency of the control group (expressing GFP only) was 82%. C, western blot analysis with antibodies against Mtb Hsp16.3; β-actin was used as a loading control.
Meng et al. BMC Microbiology 2014 14:37 doi:10.1186/1471-2180-14-37