Additional file 4.

Hla Western Blot of JKD6159, JKD6159∆hla and JKD6159∆hla r (A) Western Blot demonstrating that JKD6159∆hla does not express Hla by Western Blot and that complementation of this mutant (JKD6159∆hla r) results in restoration of Hla expression. (B) Arrangement of PCR primers used PCR screen of JKD6159∆hla and JKD6159∆hla r. (C) PCR screen of 25 randomly selected S. aureus colonies obtained from two mice (mouse 4 and mouse 7) post skin infection with JKD6159∆hla r. The PCR primers used flank the region deleted in hla for the mutant and show incomplete penetration of the bacterial population with the repaired version of hla (17/25 with an intact allele for mouse 4 and 21/25 for mouse 7), thereby explaining the inability of the repaired mutant to fully restore the virulence phenotype in this infection model.

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Chua et al. BMC Microbiology 2014 14:31   doi:10.1186/1471-2180-14-31