Figure 5.

Characterization of the genes encoding hydroxylases or oxygenases. A, LC-MS analysis (extracted ion chromatograms of m/z [M + H]+ 969.5 corresponding to PLYA) of Streptomyces sp. MK498-98F14 wild type (WT) and mutants (ΔplyE, ΔplyP, ΔplyR, Δorf4, and ΔplyM). B, LC-MS analysis (extracted ion chromatograms of m/z [M + Na]+ 975.5 and 991.5 corresponding to PLYB and PLYA) of Streptomyces sp. MK498-98F14 wild type (WT) and the ΔplyM mutant. C, LC-MS analysis (extracted ion chromatograms of m/z [M + Na]+ 959.5 corresponding to the putative biosynthetic intermediate of PLYA lacking two hydroxyl groups) of Streptomyces sp. MK498-98F14 wild type (WT) and mutants (ΔplyE, ΔplyP, ΔplyR and ΔplyM). B was performed under the conditions: 35-95% B (linear gradient, 0–20 min), 100% B (21–25 min), 35% B (25-40 min) at the flow rate of 0.3 mL/min.

Du et al. BMC Microbiology 2014 14:30   doi:10.1186/1471-2180-14-30
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