|qPCR settings and relative transcript abundance|
|Target gene||Primer sequence (5′-3′)a||Product size (bp)||PCR efficiency||Expression ratiob|
|16S rDNA||fwd: GCYTAACACATGCAAGTCGA||500||1.85||/|
aPrimer sets were designed based on the sequences of cDNA-AFLP fragments. Primers for 16S rDNA gene were designed as reported by Giraffa et al. .
bTarget gene expression was calculated relative to 16S rDNA as a reference gene using the efficiency-corrected ΔΔCT method . The relative expression ratios in CB compared to MRS are shown.
Lazzi et al.
Lazzi et al. BMC Microbiology 2014 14:28 doi:10.1186/1471-2180-14-28