Table 1

Primer and adaptor sequences
Primer/adaptor Sequence (5′-3′) Application
Adaptor EcoRI-f CTCGTAGACTGCGTACC Ligation
Adaptor EcoRI-r AATTGGTACGCAGTCTAC Ligation
Adaptor MseI-f GACGATGAGTCCTGAG Ligation
Adaptor MseI-r TACTCAGGACTCAT Ligation
EcoRI-0 GACTGCGTACCAATTC Non-selective PCR
MseI-0 GATGAGTCCTGAGTAA Non-selective PCR
5′DY-EcoRI-AT GACTGCGTACCAATTCAT Selective PCR
5′DY-EcoRI-AC GACTGCGTACCAATTCAC Selective PCR
MseI-AT GATGAGTCCTGAGTAAAT Selective PCR
MseI-AC GATGAGTCCTGAGTAAAC Selective PCR
EcoRI-AC GACTGCGTACCAATTCAC Re-amplification PCR
EcoRI-AT GACTGCGTACCAATTCAT Re-amplification PCR

Primer sets were designed as reported by Bove et al. [18].

Lazzi et al.

Lazzi et al. BMC Microbiology 2014 14:28   doi:10.1186/1471-2180-14-28

Open Data