PCN activates NF-κB signaling pathway. Differentiated U937 cells were stimulated with PCN (50 μM). At 0, 10, 30, 60, 90 and 120 min, cell proteins were collected. Cytosolic or nuclear protein was extracted, and Western blotting was performed to detect NF-κB p65 protein translocation. Levels of phospho-p65 (Ser276) in whole cell lysates (A), cytosol (B) or nuclear contents (C) were examined by Western blot analysis with the respective antibodies. Levels of p65 was also determined in nuclear fractions. β-actin was used as a control for equal loading. Data are the summary of averaged relative density units measured in 3 independent experiments. *p < 0.05 compared with control.
Chai et al. BMC Microbiology 2014 14:26 doi:10.1186/1471-2180-14-26