The expression of phosphorylated and total MAPK proteins in PMA-differentiated U937 cells. PMA-differentiated U937 cells were stimulated with PCN (50 μM) for the indicated time periods with or without pretreatment by MAPK inhibitor SB 203580 (30 μM) or PD98059 (30 μM ) for 1 h. (A and B) The expressions of phospho-ERK or ERK (A) and phospho-p38 or p38 (B). (C) The expression of phosphorylated and total p38 and ERK proteins in U937 cells. Representative data of three independent experiments are shown. **p < 0.01 compared with the A group; MAPK: mitogen-activated protein kinase; ERK: extracellular signal-regulated kinase; PMA: phorbol 12-myristate 13-acetate.
Chai et al. BMC Microbiology 2014 14:26 doi:10.1186/1471-2180-14-26