MAPK inhibitors attenuate PCN-induced IL-8 release. Different concentrations of the ERK or P38MAPK blockers (PD98059 at 10, 30, or 50 μM or SB203580 at 10, 30, or 50 μM) were added into fresh medium of PMA-differentiated U937 cells 60 min before PCN was added. Cells were exposed to PCN (50 μM) for 24 h. Supernatants were harvested for measuring IL-8 by ELISA. **p < 0.01 compared with PMA-differentiated U937 cells. MAPK: mitogen-activated protein kinase; ERK: extracellular signal-regulated kinase; PMA: phorbol 12-myristate 13-acetate.
Chai et al. BMC Microbiology 2014 14:26 doi:10.1186/1471-2180-14-26