The expression of IL-8 mRNA in PMA-differentiated U937 cells. RNA was extracted from PMA-differentiated U937 cells after various treatments (TNF-α at 10 ng/mL and PCN at 25 μM and 25 μM PCN combined 10 ng/mL TNF-α respectively) with the indicated time periods, and analyzed by RT-PCR with specific primers for IL-8. Results shown are representative of three separate experiments. Expression of IL-8 mRNA was quantified by densitometry, and standardized by the β-actin level. *p < 0.05, **p < 0.01 compared with the level at 1 h or 2 h. PMA: phorbol 12-myristate 13-acetate.
Chai et al. BMC Microbiology 2014 14:26 doi:10.1186/1471-2180-14-26