Figure 4.

Cellular and molecular responses of osteoblasts and macrophages infected withS. aureusat an MOI of 500:1 for 2 h. (A) DCF fluorescence intensity as an indicator of H2O2 production by non-infected controls and S. aureus-infected osteoblasts and macrophages. (B) DHE fluorescence intensity as an indicator of O.2 production by non-infected controls and S. aureus-infected osteoblasts and macrophages. (C) Osteoblast ALP activity measured at post-infection days 1, 4, and 7. (D) Macrophage phagocytosis activity (ingestion). Light gray area: control macrophages without incubation with S. aureus; dark gray area: non-infected macrophages; black area: infected macrophages. *p < 0.01, **p < 0.001, ***p < 0.0001, and #p < 0.05 compared to control.

Hamza and Li BMC Microbiology 2014 14:207   doi:10.1186/s12866-014-0207-5
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