THP-1 cells were incubated with different strains and mutants of FITC-labelled (green) P. gingivalis (MOI 100) for 24 hours and then stained for F-actin with rhodamine phalloidin(red) and nucleus with DAPI (blue), followed by confocal fluorosence microscopy. Confocal images show (A) Control (untreated) THP-1 cells (B) ATCC 33277 (C) ATCC 33277 heat-killed (D) W50 (E) W50 heat-killed (F) E8 and (G) K1A adhere to and are found within monocytes. Large, multi-nucleated cells are indicated by white arrows in figures B, C, D and E. Bar = 40μm. 60X objective, oil immersion. Representative images from three independent experiments are shown.
Jayaprakash et al. BMC Microbiology 2014 14:193 doi:10.1186/1471-2180-14-193