P. gingivalis degrades CXCL8 through gingipains. The culture supernatant of heat-killed W50 (MOI 100) treated THP-1 cells for 24 hours was filtered and incubated with 107/ml, 108/ml, 109/ml viable ATCC3277 (ATCC), W50, E8, K1A or heat-killed ATCC 33277 (ATCCHK) or W50 (W50HK) for 4 hours and CXCL8 was quantified by ELISA. Significant differences among each group were analyzed in relation to the control and statistics were done using one-way ANOVA. Values represent the mean ± SD. (*p < 0.05; **p < 0.01; ***p < 0.001; n = 3).
Jayaprakash et al. BMC Microbiology 2014 14:193 doi:10.1186/1471-2180-14-193