Table 2

Polyamine profile in Caco-2 cells after 6 h of exposure to viable L.GG (108CFU/ml), L.GG-HK and L.GG-CM, alone or in combination with gliadin (1 mg/ml)
Control Viable L.GG L.GG-HK L.GG-CM Gliadin Gliadin + Viable L.GG Gliadin + L.GG-HK Gliadin + L.GG-CM
Putrescine 0.15 ± 0.1a 0.12 ± 0.1a 0.1 ± 0.2a 0.12 ± 0.1a 0.2 ± 0.005a 0.2 ± 0.008a 0.16 ± 0.005a 0.2 ± 0.01a
Spermidine 6.9 ± 0.08a 3.3 ± 0.1c 3.8 ± 0.2c 6.8 ± 0.09a 9.3 ± 0.05b 6.0 ± 0.06a 7.1 ± 0.05a 8.2 ± 0.2ab
Spermine 7.8 ± 0.05a 4.3 ± 0.04c 5.3 ± 0.5c 7.5 ± 0.05a 11.1 ± 0.3b 4.3 ± 0.1c 8.9 ± 0.03a 11.3 ± 0.09 ab
Total polyamines 14.3 ± 0.3a 7.9 ± 0.5c 9.1 ± 0.6c 14.4 ± 0.5a 20.9 ± 0.8b 10.3 ± 0.4c 15.9 ± 0.3a 20.01 ± 0.5b

All data represent the results of three different experiments (mean ± SEM). For each treatment mean values not sharing a common superscript differ significantly (P < 0.05, Kruskal-Wallis analysis of variance and Dunn’s Multiple Comparison Test). Polyamines are in nmol/mg protein.

Orlando et al.

Orlando et al. BMC Microbiology 2014 14:19   doi:10.1186/1471-2180-14-19

Open Data