Antagonism analyses of C. rosea strains. A: Plate confrontation assay against B. cinerea (Uppar lane), R. solani (middle lane) and F. graminearum (lower lane). Agar plugs of C. rosea (left side in the plate) strains and B. cinerea, R. solani or F. graminearum (right side in the plate) were inoculated on opposite sides in 9 cm diameter agar plates and incubated at 25°C. The experiment was performed in 3 replicates and photographs of representative plates were taken 20 days post inoculation. B: Tolerance of C. rosea strains to the secreted metabolites of B. cinerea (Bc), R. solani (Rs) and F. graminearum (Fg). Agar plugs were inoculated on PDA plates covered with cellophane and incubated at 25°C in darkness. After reaching to the end of plate the colony was removed together with the cellophane disc. Plates were re-inoculated with a C. rosea WT, ΔHyd1, ΔHyd3, ΔHyd1ΔHyd3, and ΔHyd1+, ΔHyd3+ agar plug, incubated at 25°C and linear growth was recorded daily. C: Secretion assay of C. rosea strain. Fungal strains were grown in potato dextrose broth for 10 days at 25°C. Culture filtrates was collected after removing mycelia mass and were inoculated with B. cinerea (Bc), R. solani (Rs) or F. graminearum (Fg) agar plug. Biomass production in culture filtrates was analysed by determining mycelial dry weight post 3 days of inoculation. Error bars represent standard deviation based on 3 biological replicates. Different letters indicate statistically significant differences (P ≤ 0.05) within experiments based on the Tukey-Kramer test.
Dubey et al. BMC Microbiology 2014 14:18 doi:10.1186/1471-2180-14-18