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Open Access Highly Accessed Research article

Whole-genome sequencing reveals novel insights into sulfur oxidation in the extremophile Acidithiobacillus thiooxidans

Huaqun Yin12*, Xian Zhang12, Xiaoqi Li12, Zhili He3, Yili Liang12, Xue Guo12, Qi Hu12, Yunhua Xiao12, Jing Cong12, Liyuan Ma12, Jiaojiao Niu12 and Xueduan Liu12*

Author Affiliations

1 School of Minerals Processing and Bioengineering, Central South University, Changsha, China

2 Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha, China

3 Institute for Environmental Genomics, University of Oklahoma, Norman, OK, USA

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BMC Microbiology 2014, 14:179  doi:10.1186/1471-2180-14-179

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2180/14/179


Received:14 February 2014
Accepted:19 June 2014
Published:4 July 2014

© 2014 Yin et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Abstract

Background

Acidithiobacillus thiooxidans (A. thiooxidans), a chemolithoautotrophic extremophile, is widely used in the industrial recovery of copper (bioleaching or biomining). The organism grows and survives by autotrophically utilizing energy derived from the oxidation of elemental sulfur and reduced inorganic sulfur compounds (RISCs). However, the lack of genetic manipulation systems has restricted our exploration of its physiology. With the development of high-throughput sequencing technology, the whole genome sequence analysis of A. thiooxidans has allowed preliminary models to be built for genes/enzymes involved in key energy pathways like sulfur oxidation.

Results

The genome of A. thiooxidans A01 was sequenced and annotated. It contains key sulfur oxidation enzymes involved in the oxidation of elemental sulfur and RISCs, such as sulfur dioxygenase (SDO), sulfide quinone reductase (SQR), thiosulfate:quinone oxidoreductase (TQO), tetrathionate hydrolase (TetH), sulfur oxidizing protein (Sox) system and their associated electron transport components. Also, the sulfur oxygenase reductase (SOR) gene was detected in the draft genome sequence of A. thiooxidans A01, and multiple sequence alignment was performed to explore the function of groups of related protein sequences. In addition, another putative pathway was found in the cytoplasm of A. thiooxidans, which catalyzes sulfite to sulfate as the final product by phosphoadenosine phosphosulfate (PAPS) reductase and adenylylsulfate (APS) kinase. This differs from its closest relative Acidithiobacillus caldus, which is performed by sulfate adenylyltransferase (SAT). Furthermore, real-time quantitative PCR analysis showed that most of sulfur oxidation genes were more strongly expressed in the S0 medium than that in the Na2S2O3 medium at the mid-log phase.

Conclusion

Sulfur oxidation model of A. thiooxidans A01 has been constructed based on previous studies from other sulfur oxidizing strains and its genome sequence analyses, providing insights into our understanding of its physiology and further analysis of potential functions of key sulfur oxidation genes.

Keywords:
Acidithiobacillus thiooxidans; Whole genome sequence; Bioinformatics analysis; Real-time quantitative PCR; Sulfur oxidation model

Background

Acidithiobacillus thiooxidans (A. thiooxidans), an extremely acidophilic, chemolithoautotrophic, gram-negative, rod-shaped microorganism, which are typically related to copper mining operations (bioleaching), has been well studied for industry applications. A. thiooxidans grows and survives by autotrophically utilizing elemental sulfur and reduced inorganic sulfur compounds (RISCs) as energy source [1], but it cannot use energy or electrons acquired from the oxidation of ferrous iron (Fe (II)) for carbon dioxide fixation as well as other anabolic processes [2].

Previous studies showed that the oxidation of elemental sulfur and RISCs was found in various strains of Acidithiobacillus ferrooxidans (A. ferrooxidans) through the detection of several enzymatic activities [3,4], but some of these activities were not associated with specific genes. The studies of oxidation and electron transfer pathways for elemental sulfur or RISCs are more complicated than those for ferrous iron, making the gene prediction and pathway clarification much more difficult [5]. Furthermore, some steps independent of enzymatic catalysis took place spontaneously, which adds the difficulty to modeling the mechanism of such a pathway. Fortunately, the method based on genome sequence analysis could provide the opportunities to predict some of these missing assignments, and also to suggest novel genes involved in the oxidation of elemental sulfur or RISCs such as sulfide, thiosulfate, and tetrathionate [6].

Elemental sulfur exists in the form of a stable octasulfane ring (S8) in nature, which forms orthorhombic crystals with extremely poor water solubility [7]. An activation prior to oxidation was postulated [6,8]. The first and critical step of sulfur oxidation could be an opening of the S8 ring by the thiol groups of cysteine residues, resulting in the formation of thiol-bound sulfane sulfur atoms (R-S-SnH) [8,9]. Subsequently, the R-S-SnH is transported into the periplasm and then oxidized by sulfur dioxygenase (SDO), of which gene(s) has (have) not yet been detected [6,9,10].

As sulfide was found to be one of the most common forms of S in the inorganic sulfur compounds, the initial step in RISCs oxidation mainly was the transition of sulfide into S0[11], forming a conjugated sulfur compound bound to a membrane fraction [5]. Furthermore, the first step of hydrogen sulfide oxidation is its conversion to sulfur or polysulfide in many phototrophic and chemotrophic bacteria by flavocytochrome c (Fcc), or by sulfide quinone reductase (SQR), which are located in the periplasm and the periplasmic surface of the cytoplasmic membrane, respectively [11,12]. Moreover, Fcc does not exist in various sulfide-oxidizing bacteria and appears to be restricted to certain species which possess the ability of thiosulfate oxidation [13].

Compared with the enzymes described above, the sulfur oxidizing (Sox) system has been elaborated in facultatively lithoautotrophic Paracoccus pantotrophus (P. pantotrophus). It is located in the periplasm and comprised of SoxXA (both c type cytochromes), SoxYZ (covalently sulfur-binding protein and sulfur compound chelating protein, respectively), SoxB (monomeric, dimanganese-containing protein that is similar to zinc-containing 5′-nucleotidases and considered to act as the sulfate thiol esterase component of the Sox system), and Sox (CD)2 (sulfur dehydrogenase), which mediate hydrogen sulfide-, sulfur-, thiosulfate- and sulfite-dependent cytochrome c reduction [14-17]. In contrast to the P. pantotrophus, the Sox (CD)2 complex is absent in the truncated Sox system of many α-Proteobacteria [17].

The periplasmic thiosulfate is synthesized spontaneously from sulfite and a sulfur atom [9], and then catalyzed to generate tetrathionate by thiosulfate:quinone oxidoreductase (TQO), which is constituted of a large subunit (DoxD) and a smaller subunit (DoxA); tetrathionate is then hydrolyzed by tetrathionate hydrolase (TetH) to produce thiosulfate and other uncertain products. TetH was studied previously in A. ferrooxidans[18], and the tetH gene cluster has been also characterized in Acidithiobacillus caldus (A. caldus) [19].

In addition, other RISCs oxidation enzymes were identified in Acidithiobacillus spp. including: rhodanese or thiosulfate sulfurtransferase (TST) and heterodisulfide reductase (HDR). The TST widely exists in the cytoplasm of both prokaryotes and eukaryotes. It cleaves the sulfur-sulfur bond of thiosulfate to yield sulfur and sulfite, and then the former is transferred to a thiophilic acceptor such as cyanide and thiol compounds [20,21]. The cytoplasmic heterodisulfide reductase complex HdrABC was reported to catalyze the reversible reaction of the disulfide bond X-S-S-X reduction accompanied with energy conservation in sulfate reducing archaea and bacteria and methanogenic archaea [2], while this complex was only speculated from transcriptomics and genomics analysis instead of biochemical experiments in A. ferrooxidans[9].

Recently, a model of electron transfer pathways involved in the sulfur oxidation of A. ferrooxidans was proposed, in which electrons released from RISC oxidation were transferred either to terminal oxidases to produce proton gradient or to NADH complex I to generate reducing power via the quinol pool (QH2) [2,6]. However, the lack of genetic manipulation systems has greatly restricted the exploration of the molecular biology and physiology in extreme acidophilic microorganisms, and considerably less information is known about the mechanism by which microorganisms grow, survive and proliferate in extremely acidophilic environments. With the ongoing and rapid development of sequencing technologies and the continuous improvement of bioinformatics-based analytical methods, effective tools have been offered for investigating metabolic and regulatory models [22]. A substantial body of information could be acquired by deep genome analysis, which can assist the laboratory scientists to focus on experimental investigation of several most significant predictions, thus save considerable time and efforts [23].

To get a better understanding of how these metabolic processes occur and further explore how to make them more efficient in A. thiooxidans, the whole-genome sequencing was carried out. Through bioinformatics analysis of the bioleaching bacterium, A. thiooxidans genome sequence, it is expected that we would predict and validate the genes and conserved gene clusters involved in sulfur oxidation. Subsequently, a further experiment at the transcriptional level was performed via quantitative real-time PCR (qRT-PCR). On the basis of bioinformatics analysis, together with qRT-PCR data, a putative model of sulfur oxidation in A. thiooxidans was proposed.

Methods

Ethics statement

The strain (A. thiooxidans A01) was obtained from a wastewater of coal dump of Jiangxi, China. This study doesn’t involve any ethical issue.

Bioinformatics analysis of A. thiooxidans genome sequence

A bioinformatics pipeline was used to analyze the genome sequence of A. thiooxidans. The genomic DNA of A. thiooxidans A01 was extracted using TIANamp Bacteria DNA Kit (TIANGEN) according to the manufacturer’s instructions and then sequenced by BGI- Shenzhen (Beijing Genomics Institute) using Illumina HiSeq 2000 for 2 × 100 bp paired-end sequencing (Illumina, Inc. USA). After filtering, with Phred 20 as a cutoff, high quality raw sequences were assembled into longer fragment sequences, contigs and scaffolds, relied on strategy using SOAPdenovo version 2.0 [24]. According to previous data, coding regions detection and potential genes identification were performed using Glimmer [25]. Moreover, RepeatMasker [26] was used to screen DNA sequences with interspersed repeats and low complexity. The RNAmmer [27] and tRNAscan-SE [28] were used to search for rRNA genes and tRNA genes in genomic sequence, respectively.

To further analyze the candidate genes and their predicted protein products, perl scripts written in our laboratory were used to extract the corresponding sequences of previously predicted CDSs. Subsequently, each putative gene was annotated using the BLASTx program (e value, ≤1e-5) against the alternative database such as Non-redundant protein database, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Clusters of Orthologous Groups of proteins (COG).

Media and culture conditions

The strain A. thiooxidans A01 was isolated in this laboratory. The components of 9 K medium [29] and DSMZ medium 71 [30] corresponding to S0 and Na2S2O3 media respectively were previously described in references. Elemental sulfur (S0) (boiling sterilized, 10 g/L) and Na2S2O3 · 5H2O (sterile filtration, 5 g/L) were added as substrates prior to inoculation. The bacterium was cultivated in 100-ml culture medium with an initial pH 2.0 for 9 K medium and pH 4.4 for DSMZ medium 71. The initial bacterial concentration was 2.5 × 106 cells/ml and the cultivation temperature was 30°C. The shaking speed for liquid cultivation of A. thiooxidans A01 was 170 rpm if not specifically stated. All cultures under the same conditions were manipulated in triplicate.

Quantitative real-time PCR (qRT-PCR)

Primers targeting selected genes putatively involved in sulfur oxidation were designed for quantitative real-time RT-PCR (product size 114–270 bp; Table 1). Cells were collected by centrifugation from 100 ml medium at the exponential growth phase (54 h) and washed twice using sterile RNase-free ddH2O. RNA was extracted using the Trizol Reagent method [31]. Total RNA extract was purified using MicroElute RNA Clean-Up Kit (OMEGA) in accordance with the manufacture’s recommendations, and the digestion of contaminating DNA was performed with RNase-free DNase I (OMEGA) to remove genomic DNA. RNA concentration and purity was measured at OD260 and OD280 with a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies).

Table 1. Primers used in qRT-PCR detection of genes related to sulfur oxidation

Subsequently, the total RNA of 2 μg was reversely transcribed using First Strand cDNA Synthesis Kit (TOYOBO) under the following conditions: 30°C for 10 min, 42°C for 20 min, 99°C for 5 min, and 4°C for 5 min. RT reaction products of 1 μl as template in 25 μl reaction volume were used for PCR amplification with specific primers (Table 1) using QuantiFast SYBR Green PCR Kit (QIAGEN). The conditions for the PCR reaction were as follows: 95°C for 5 min followed by 40 cycles at 95°C for 30 s, 56°C for 15 s and 72°C for 25 s in MyiQ Single-Color Real-Time PCR Detection System (BIO-RAD). The transcription reference gene, glyceraldehyde-3-phosphate dehydrogenase gene (gapdh), was used for normalization. The relative fold changes in gene expression were calculated using the 2-ΔΔCT method [32].

Nucleotide sequence accession numbers

The draft genome sequence of A. thiooxidans A01 was deposited at DDBJ/EMBL/GenBank under the accession number AZMO00000000. The version described in this paper is version AZMO01000000.

Results and discussion

Genomic properties

According to our sequencing data, the draft genome of A. thiooxidans A01 contains 3,820,158 total base pairs with GC content of 53.08% distributed in 213 contigs (Table 2). The maximum contig length is 259,764 bp, and the minimum length is 201 bp. The contig length has an N50 length of 46,830 bp. Compared to the draft genome of A. thiooxidans ATCC 19377 (AFOH00000000) in the NCBI database, the draft genome of A. thiooxidans A01 has much larger size, which indicates more information for gene prediction. In addition, 111 tRNA, one 5S-16S-23S operon and 3,660 protein-coding sequences (CDSs) were predicted. As to the 3,660 CDSs, 2,537 were assigned a putative function in the current databases, 136 were conserved hypothetical protein, and 987 were hypothetical proteins. Also, a total of 3,361 were involved in the KEGG pathways, and 2,664 were involved in the clusters of orthologous groups of proteins (COGs).

Table 2. General features of draft genome sequence of A. thiooxidans A01

Predicted genes involved in sulfur oxidation

Sulfur oxidation with sulfide, sulfur, sulfite, thiosulfate and tetrathionate as various oxidation states is the main pathway from a complete oxidation of sulfide to sulfate [33]. Based on genome sequence analysis, genes predicted to be involved in the oxidation of elemental sulfur and RISCs and electron transfer were detected in the genome (Table 3). It reveals that most of putative genes for sulfur oxidation [sqr, sor, two copies of soxABXYZ, hdrABC, doxD, tetH and rhodanese (rhd)] in A. thiooxidans also exist in A. caldus[11] and other microbial representatives derived from extreme acidic environments. Especially, the candidate genes potentially encoding sulfur dioxygenase (SDO) were detected in A. thiooxidans A01. In addition, A. thiooxidans also has genes encoding phosphoadenosine phosphosulfate (PAPS) reductase and adenylylsulfate (APS) kinase.

Table 3. Amino-acid sequence identities of the products encoded by sulfur metabolic genes between A. thiooxidans A01 and other thiobacteria including A. ferrooxidans (CP001132), A. caldus (CP002573) and T. denitrificans (CP000116)

The first documented step in elemental sulfur oxidation is the transition of sulfur to thiosulfate, which is catalyzed by SDO. In A. thiooxidans, three putative sdo orthologs located at the draft genome sequence (contig8: 62452–63315, contig50: 14318–15058 and contig97: 6229–6966) belong to the large and considerably variable metallo-beta-lactamase superfamily (cl00446), which have the signature motif H-X-H-X-D-H (Figure 1). Previous research revealed that SDO in Urechis unicinctus (AEV92813) possessed the conserved metal I binding sites (H113, H115, H169 and D188), metal II binding sites (D117, H118, H169 and H229) and potential glutathione (GSH) binding sites (R197, Y231, M279 and I283) [34]. As is depicted in Figure 1, their conserved sites in At-SDO are much similar to AEV92813, and the possible reason why GSH binding sites between them is slightly different may be their distant relationship. Thus, the conserved regions observed with U. unicinctus as well as SDOs from other species possibly indicate the similar functional properties. However, the properties of SDO-like protein in A. thiooxidans are required further studies. Another gene encoding sulfide quinone reductase (SQR) was detected in the draft genome sequence of A. thiooxidans A01, and the product of sqr gene shares 79% and 72% identity with other sqr ortholog identified in A. ferrooxidans and A. caldus, respectively (Table 3).

thumbnailFigure 1. Multiple sequence alignment among the SDO sequences from various species. Conserved metal ion binding sites and potential GSH binding site are indicated by * and ■, respectively. The signature motif of metallo-beta-lactamase superfamily is marked with a red rectangle. GenBank accession number: Homo sapiens (NP_055112); Mus musculus (NP_075643); Urechis unicinctus (AEV92813); Caenorhabditis elegans (NP_501684); Apis mellifera (XP_393510); Acidovorax delafieldii (ZP_04761469); Acidithiobacillus caldus (YP_004749948); Nitrosococcus watsonii (YP_003760989); Methylobacter tundripaludum (ZP_08782165).

Other enzymes reported to be involved in sulfur oxidation are TQO and TetH. Our sequence analysis revealed that the homolog of doxDA existed in the draft genome of A. thiooxidans A01, and it is predicted to encode a thiosulfate:quinone oxidoreductase (TQO), and also has a conserved DoxD domain (pfam04173) and a conserved DoxA domain (pfam07680). There is a fusion of separate DoxD- and DoxA–like subunits that were reported previously in A. ferrooxidans DoxD [6]. The putative TetH of A. thiooxidans shares 60% and 77% identity with TetH in A. ferrooxidans and A. caldus respectively, indicating their high similarity in orthologous relationship. Our analysis also indicates that TetH of all sequenced Acidithiobacillus spp. has a conserved pyrrolo-quinoline quinone (PQQ) domain (pfam01011). Although TetH was predicted to be external membrane proteins, experimental evidence showed that it was a soluble periplasmic homo-dimer with an optimum pH of 3 in A. caldus[35]. Previous studies have revealed that there is a tetH gene cluster in A. caldus[19], which is comprised of five cotranscribed genes, tpase1, rsrR, rsrS, tetH and doxD. While in the draft genome sequence of A. thiooxidans A01, only tetH and doxD located at the upstream constitute the tetH cluster (Figure 2A).

thumbnailFigure 2. Comparison of tetH gene cluster (A) and the sox gene clusters (B) between A. thiooxidans A01 and other sulfur oxidizers containing identified or putative genes. Abbreviations: tpase, transposase; rsrR, response regulator (two-component system); rsrS: histidine kinase (two-component system); tetH, tetrathionate hydrolase; doxD, thiosulfate:quinone oxidoreductase; hyp, hypothetical protein; resB, cytochrome c-type biogenesis protein ResB; resC, cytochrome c-type biogenesis protein ResC. The tetH cluster in AT: A. thiooxidans A01, AC: A. caldus SM-1 (CP002573); Two sox clusters in AT: A. thiooxidans A01, AC: A.caldus SM-1 (CP002573), TD: T. denitrificans ATCC 25259 (CP000116). The homology proteins are expressed by the same color, and percentage of amino acid similarity is indicated. The direction of transcription is represented by the arrows.

In addition, bioinformatics analysis identified a truncated Sox sulfur oxidizing system in A. thiooxidans A01, which contains two sox gene clusters, sox cluster I (resC-soxAX-resB-hyp-soxBZY) and sox cluster II (soxXYZA-hyp-soxB), and both are quite differ from those of Paracoccus denitrificans, Pseudaminobacter salicylatoxidans and Starkeya novella: soxRSVWXYZABCDEFGH (X79242), soxGTRSVWXYZABCD (AJ404005) and soxFDCBZYAXWV (AF139113) [14,15,36,37]. Comparison of all genes sequences in two sox clusters of A. thiooxidans A01 to those of Thiobacillus denitrificans ATCC 25259 (CP000116) revealed amino-acid sequence identities in the range of 35% to 51%. Unlike the sox cluster II of A. thiooxidans A01, however, there is only additional soxXA in the genome sequence of T. denitrificans ATCC 25259 [37]. Interestingly, significant sequence similarity (52% to 88%) and relatively conserved gene constitution of A. thiooxidans A01 sox gene clusters to those of A. caldus SM-1 (CP002573) indicated the similar functional properties (Figure 2B).

Furthermore, heterodisulfide reductase (HDR) has been postulated to be involved in sulfur oxidation. All genes (hdrA, hdrB and hdrC) of HDR were found in the draft genome of A. thiooxidans A01. The putative HdrA subunit is flavoprotein within a FAD binding domain and a NAD (P)-binding Rossmann-like domain. The HdrB subunit contains the typical cysteine-rich domain and the remaining HdrC subunit contains 4Fe-4S dicluster domain. In addition, all putative Hdr subunits in A. thiooxidans A01 suggested over 90% identities to the respective Hdr subunits compared with A. ferrooxidans and A. caldus (Table 3).

Five genes encoding rhodanese (sequence length 109 aa to 155 aa) were detected and one of them was used to design primer for qRT-PCR (Table 1). The phosphoadenosine phosphosulfate (PAPS) reductase and adenylylsulfate (APS) kinase, which consecutively oxidize sulfite to produce sulfate via an indirect pathway, were found in the genome of A. thiooxidans. Two genes encoding APS kinase and three genes encoding PAPS reductase were predicted in the draft genome but their roles in sulfur oxidation remain to be established.

The genome information showed that A. thiooxidans A01 contains two gene clusters potentially encoding components of the NADH quinone-oxidoreductase complex (nuoABCDEFGHIJKLMN) similar to that in A. caldus[11]. In addition, seven copies of bd ubiquinol oxidase genes (cydAB) and two gene clusters encoding bo3 ubiquinol oxidase (cyoBACD) exist in A. thiooxidans A01. However, components of the aa3-type cytochrome oxidase genes (coxBACD) only exist in the A. ferrooxidans[6].

The sulfur oxygenase reductase gene (sor) found in A. thiooxidans

As the initial enzyme in the aerobic sulfur oxidation of thermophilic archaea [38,39] and acidophilic bacteria [40,41], the sulfur oxygenase reductase (SOR) has been identified. SOR simultaneously catalyzes elemental sulfur to produce sulfite, thiosulfate, and sulfide [38,42], which are oxygen-dependent disproportionation reactions. The catalytic reaction has certain characteristics: (1) The enzyme involved in this reaction is soluble and located in cytoplasm [43]; (2) The optimum pH and temperature for activity are founded to be 7.0 ± 0.5 (Sulfolobus brierleyi) and 65–85°C (Acidianus tengchongensis and Acidianus ambivalens), respectively [44,45]; and (3) It does not require cofactors or external electron donors/acceptors for activity [46,47].

The sulfur oxidation system based on the sulfur oxygenase reductase was only reported in several acidophilic and thermophilic archaea (e.g., A. ambivalens, A. tengchongensis) or bacteria (e.g., A. caldus), but not in the species of A. ferrooxidans and A. thiooxidans[9,17,41,48]. However, our annotations show that a potential gene encoding SOR with very similar amino acid sequence in A. caldus, was detected in A. thiooxidans A01. Interestingly, the sor gene was not found in the draft genome sequence of A. thiooxidans ATCC 19377 [49]. To date, it is unclear whether sor gene exists or not in A. thiooxidans ATCC 19377 unless the complete genome sequence is obtained. Subsequently, homology search was performed with BLASTx, and sequence analysis indicated that the putative enzyme of A. thiooxidans A01 shared 80% identity to the SOR of A. caldus. Moreover, the phylogenetic tree, which included almost all homologs of SOR derived from BLASTp search showed that SOR from A. thiooxidans was detected to be closest to that isolated from A. caldus. High bootstrap values insure the reliability of clustering (Figure 3).

thumbnailFigure 3. Phylogenetic dendrogram based on the SORs from A. thiooxidans and its close homologs from other species. It was constructed using the neighbor-joining algorithm implemented in the MEGA version 5.05, and its reliability was evaluated by 1,000 bootstrap replicates.

In addition, homology searches showed that the nucleotide sequence of this putative gene in A. thiooxidans and its predicted amino acid sequence have high similarities to other species. Multiple sequence alignment of SORs from A. thiooxidans and other species was carried out (Figure 4). It is demonstrated that three cysteine residues located in two separately conserved domains, C32 at V-G-P-K-V-C32 and C102 and C105 at C102-X-X-C105, are essential to its activity [50]. In addition, the conserved motif H87-X3-H91-X23-E115, which is considered to be iron binding site [47], is detected in SOR from A. thiooxidans. The crystal structure of the SOR in A. ambivalens is demonstrated to be a large homo-multimer composed of 24 identical monomers of 308 residues, forming a large hollow sphere [51,52]. The active sites of SOR are constituted of a mononuclear non-heme iron site and three conserved cysteine residues [45]. Furthermore, the structural analysis has been performed to study the potential functions of the cysteine residues in A. tengchongensis. It is proposed that C32 residue constitutes most possibly the substrate binding site and that C102 and C105, together with the iron binding motif H87-X3-H91-X23-E115, probably form the catalytic site.

thumbnailFigure 4. Multiple sequence alignment of SORs between A. thiooxidans and other species. Protein sequences were aligned by the DNAMAN multiple sequence alignment program. The putative domains in SOR were boxed by the rectangle. And the conserved domains V-G-P-K-V-C32, H87-E-E-M-H91 and C102-XX-C105 were indicated by the rectangleA, B, C, respectively. Abbreviations: A. thiooxidans, Acidithiobacillus thiooxidans; A. caldus, Acidithiobacillus caldus (WP_004871908); A. ferrivorans, Acidithiobacillus ferrivorans (YP_004785009); A. ambivalens, Acidianus ambivalens (P29082); A. tengchongensis, Acidianus tengchongensis (AAK58572); S. tokodaii, Sulfolobus tokodaii (NP_377053); S. thermosulfidooxidans, Sulfobacillus thermosulfidooxidans (WP_020375642).

So far as we know, the sor gene is for the first time detected and reported in A. thiooxidans. The discovery of sor gene supplies a novel idea that these genome-based predictions can offer new opportunities to detect similar genes in other microorganisms and also provide new markers to explore the metabolic pathways. However, the further biochemical experiments at the transcription and protein levels should be carried out in order to verify the presence or absence of sor gene.

Growth of A. thiooxidans in S0 and Na2S2O3 media

To examine the growth of A. thiooxidans A01, Starkey-S0 or Starkey-Na2S2O3 was used as the substrate in liquid media. The results showed that A. thiooxidans had the ability to utilize both S0 and Na2S2O3 as the energy sources (Figure 5). Furthermore, the soluble Na2S2O3 was used prior to S0 and bacteria in the Na2S2O3 medium reached stationary phase earlier than that in the S0 medium. Moreover, the cell concentration of A. thiooxidans in the Na2S2O3 medium was obviously higher than that in the S0 medium, suggesting that A. thiooxidans has a highly efficient thiosulfate oxidizing ability to enable it to grow better with Na2S2O3 as substrate. One of possible reasons is that Na2S2O3 can more easily and quickly enter into the organism and then be used as energy source, while S0 needs to be activated before it is transferred into the periplasm, resulting in a slower growth and lower cell concentration with S0 as substrate.

thumbnailFigure 5. The growth curve of Acidithiobacillus thiooxidans A01 in S0 medium and Na2S2O3 medium. Data are average of three growth experiments.

Expression of selected genes involved in sulfur oxidation

To understand how those identified key genes involved in sulfur oxidation are expressed with Na2S2O3 and S0 as substrates, we further examined the expression of the selected sulfur oxidation genes with Na2S2O3 as substrate compared to those with S0 as substrate using qRT-PCR (Table 4). The gapdh gene was used as the internal control to regulate the systematic and random errors during the process of operation procedure. Statistical significance was assessed by the Student’s t-test, and the gene expression levels with fold change ≥ 1.50 and p-value ≤ 0.05, or with fold change ≤ 0.67 and p-value ≤ 0.05 were up-regulated and down-regulated, respectively. Our results showed that all assayed genes were transcribed when grown in S0 or Na2S2O3, and t-test analysis revealed the significant (p < 0.05) differences in gene expression between S0- and Na2S2O3-grown cells were observed. Although no obvious changes at the transcription level were detected for tetH, hdrA and the sox gene cluster II (e.g., soxA-II, soxB-II and soxY-II), the other genes (e.g., sqr, sor and tqo) were down-regulated at various degrees with Na2S2O3 as substrate (Table 4). Based on the growth curve and qRT-PCR data, it is speculated that soluble Na2S2O3 could quickly enter into the cell and then stimulate the up-regulation of genes involved in sulfur oxidation at the early stage, whereas the accumulation of products derived from sulfur oxidation in turn might inhibit the process of enzyme reactions and then influence the gene expression. With S0 as substrate, however, elemental sulfur needs to be activated before through the outer membrane, and the majority of sulfur oxidation genes including sor gene, which was detected to be the low expression level at the early growth phase in A. caldus[9], would play an important role till the mid-log phase.

Table 4. Comparison of raw fold changes in gene expression between S0 and Na2S2O3-grown cells (mid-log phase) obtained by qRT-PCR in this study

With S0 in the medium, a mass of sulfur atoms (S) and hydrogen sulfide are produced during the activation of S8 and then are used as the substrates of SQR, SOR, TST and HDR in succession. This could be the reason for the higher expression of sqr, sor, rhd and hdrABC in the S0 medium than in the Na2S2O3 medium (Table 4). Our experiments showed that thiosulfate was sufficient at the mid-log phase in the Na2S2O3 medium, thus resulting in the lower expression level of doxD and tetH. The sox operon I but not sox operon II together with a bo3 ubiquinol oxidase operon gene may make it feasible to regulate and control at their transcriptional level, thus this may be one of the reasons why the expression of the sox operon I was up-regulated in the S0 medium whereas the sox operon II had no obvious changes in these two substrates.

Construction of sulfur oxidation model in A. thiooxidans

In order to acquire the functional attributes of cells, it is necessary to understand the structural constitution and characters of cellular metabolic networks [53]. With respect to sulfur oxidation, a bioinformatics analysis of the genome sequence of organism was performed, indicating that various enzymes, enzyme complexes, and the electron transport chain components were located in different cellular compartments. Based on the documented models in other Acidithiobacillus species [2,6,9-11], genome sequence analysis, our current knowledge, and experimental results in this study, a hypothetical model is developed for sulfur oxidation in A. thiooxidans A01 (Figure 6).

thumbnailFigure 6. The sulfur oxidation model in A. thiooxidans based on documented models [[2],[6],[9]-[11]], bioinformatics analysis of draft genome sequence. Abbreviations: SDO, sulfur dioxygenase; SQR, sulfide quinone reductase; TQO, thiosulfate:quinone oxidoreductase; TetH, tetrathionate hydrolase; Sox, sulfur oxidizing protein; HDR, heterodisulfide reductase; SOR, sulfur oxygenase reductase; TST, thiosulfate sulfurtransferase; A, phosphoadenosine phosphosulfate reductase; B, adenylylsulfate kinase. As is shown, the sulfur oxidation metabolism in A. thiooxidans A01 contains various sulfur oxidation systems and the electron transfer pathways in different cellular compartments. (i) In the outer membrane, elemental sulfur (S8) in the form of stable octasulfane ring is activated and transported into the periplasm as thiol-bound sulfane sulfur atoms (R-S-SnH); (ii) In the periplasm, R-S-SnH is oxidized by SDO, while tetrathionate is utilized by TetH to produce thiosulfate, and the thiosulfate is sequentially catalyzed by Sox complex; (iii) In the cytoplasmic membrane, SQR and TQO carry out their respective functions with electrons further transferred to terminal oxidase or NADH complex I; and (iv) In the cytoplasm, the particular enzymes perform the catalytic reaction by a sequence of steps that eventually produce sulfate.

The first documented step in sulfur oxidation system is the activation of extracellular elemental sulfur (S8) to thiol-bound sulfane sulfur atoms (R-S-SH) and then it is transferred into the periplasm where it is oxidized by the sulfur dioxygenase (SDO) to produce sulfite (Table 5), accompanied with the generation of hydrogen sulfide. Subsequently, hydrogen sulfide could be converted to sulfur atoms (S) by sulfide quinone reductase (SQR) located in the cytoplasmic membrane. The sulfur atoms could be accumulated to form polymeric sulfur (Sn), and then transferred via an unknown mechanism into the cytoplasm where it is catalyzed by sulfur oxygenase reductase (SOR). The possible products of disproportionation reaction performed by SOR are sulfide, thiosulfate and sulfite. Sulfide could be converted to hydrogen sulfide and then oxidized by SQR, while thiosulfate is considered to be used as the substrate of rhodanese (TST) to produce sulfite and sulfur atom. Subsequently, the cytoplasmic thiol protein (RSH) that is acted as sulfur atom acceptor obtains a sulfur atom to form sulfane sulfate (RSSH), and then the latter is catalyzed by the heterodisulfide reductase complex (HDR) to regenerate RSH. Therefore, a cycle relied on TST and HDR is proposed: RSH acquires a sulfur atom derived from the catalysis of thiosulfate, which is catalyzed by TST, to generate RSSH, and then RSSH is used as the substrate of HDR to reproduce RSH [9].

Table 5. Enzyme properties involved in the sulfur oxidation of A. thiooxidans

In addition, sulfite could have pernicious effect on the cell unless it is digested quickly in the organism. So far, the sulfite acceptor oxidoreductase (SAR) that catalyzes sulfite to sulfate has not been identified in the genome of A. thiooxidans A01, neither was the A. thiooxidans DSM 17318 genome [10]. Phosphoadenosine phosphosulfate (PAPS) reductase and adenylylsulfate (APS) kinase genes were discovered in A thiooxidans A01, while the sulfate adenylyltransferase dissimilatory-type (SAT) gene that catalyzed adenosine - 5′ - phosphosulfate (APS) to sulfite was not detected (Figure 6). Therefore, another putative pathway based on PAPS reductase and APS kinase that catalyze sulfite to produce sulfate indirectly is proposed: sulfite is catalyzed by PAPS reductase and APS kinase to generate PAPS and APS in succession, while the latter could produce sulfate as the final product via an unknown mechanism.

Another putative pathway of sulfur oxidation is that the periplasmic sulfite combines with sulfur atom to form thiosulfate spontaneously, without enzymatic catalysis. Thiosulfate:quinone oxidoreductase (TQO) located in the cytomembrane is responsible for the catalysis of thiosulfate to produce tetrathionate. The thiosulfate oxidation catalyzed by TQO was illustrated previously in A. ambivalens: DoxD catalyzes thiosulfate to tetrathionate and simultaneously produces two electrons, and then DoxA transfers electrons to the quinone [54]. Tetrathionate hydrolase (TetH), a soluble periplasmic enzyme, is able to catalyze the hydrolysis of tetrathionate [35]. The documented hydrolysates of tetrathionate in A. thiooxidans are thiosulfate and sulfate [55]. Furthermore, sulfur atoms (S) may be also one of important hydrolysates, which explains why the expression level of sor gene was higher in S0 medium.

Thiosulfate could be directly catalyzed by the incomplete Sox complex to generate sulfate [10]. As to the truncated Sox system which is absent of Sox (CD)2, the well-studied pathways were provided from some valuable references [9,17,56,57]. Initially, the SoxXA complex oxidatively couples the sulfane sulfur of thiosulfate to a SoxY-cysteine-sulfhydryl group of the SoxYZ complex to form SoxY-thiocysteine-S-sulfate (SoxYZ-S-S-SO3). Subsequently, the terminal sulfone (−SO3) group is released as sulfate by the activity of the SoxB component to produce S-thiocysteine (SoxYZ-S-S). Due to the lack of the sulfur dehydrogenase Sox (CD)2 component, the sulfur atom of the sulfane intermediate (SoxYZ-S-S) is plausibly dropped from SoxYZ or oxidized by the alternative sulfur dioxygenase (SDO) to generate SoxYZ-cysteine-S-sulfate (SoxYZ-S-SO3) [9]. Eventually, the sulfonate moiety of SoxYZ-S-SO3 is again hydrolyzed by SoxB, regenerating SoxYZ in the process. In addition, other forms of sulfur except for thiosulfate could participate in the Sox pathway via either enzymatic (such as HS) or nonenzymatic (such as S8) conjugation to SoxY at the proper intermediate state [17].

There is a strong connection between the expression of the sox cluster genes and the terminal oxidase genes. The hypothetical electron pathway is that electrons from the Sox system are transferred via QH2 to the terminal oxidases (bd and bo3) and the NADH complex (Figure 6).

To date, little is known about electron transfer chains in A. thiooxidans, but it has been elaborated in A. ferrooxidans. One of the electron transfer chains in A. ferrooxidans is that electrons from ferrous iron oxidation flow through Cyc2 to rusticyanin, and then be transferred to oxygen via c-cytochrome Cyc1 to aa3 cytochrome oxidase (downhill electron pathway) or to NAD+ via c-cytochrome CycA1 → bc1 complex → ubiquinone pool → NADH dehydrogenase (uphill electron pathway) [2,6,58]. Another is that electrons from elemental sulfur or RISCs are transferred via the quinol pool (QH2) either (1) directly to terminal oxidases bd or bo3, or indirectly to a bc1 complex and a cytochrome c (CycA2) or a high potential iron-sulfur protein (HiPIP), whose gene iro was identified to be associated with the pet II gene cluster thought to be involved in sulfur oxidation, probably to the aa3 oxidase to produce a proton gradient or (2) to NADH complex I to generate reducing power [2,6]. However, A. thiooxidans has only the sulfur oxidation system and annotated results showed that bo3-type terminal oxidases and bd-type terminal oxidases exist in the draft genome of A. thiooxidans A01. Thus, the hypothesized electron transfer chain in A. thiooxidans A01 is proposed: electrons from SQR, TQO, Sox compounds and HDR are transferred through the QH2 either to terminal oxidase (bd and bo3) to produce proton gradient, or to NADH complex I to generate reducing power.

Conclusion

Bioinformatics analysis of the genome sequence of A. thiooxidans A01 provides a valuable platform for gene discovery and functional prediction that is much important given the difficulties in performing standard genetic research in this microorganism. Based on our analysis and available documented data, a hypothetical model for sulfur oxidation and electron transportation is proposed with several distinguished features. The elemental sulfur (S8) in the outer membrane is activated and transported into the periplasm as thiol-bound sulfane sulfur atoms (R-S-SnH). And then, the R-S-SnH is further oxidized in the periplasm where SDO, TetH, and Sox system perform their functions. The cytoplasmic membrane involving SQR and TQO is the third region with electrons transferring. In the cytoplasm, the sulfur-containing metabolites are catalyzed to eventually produce sulfate by a series of enzymes. Therefore, this study provides novel insights and more instructive guides into sulfur oxidation metabolism in A. thiooxidans. However, many fundamental questions remain unanswered. For example, some genes involved in the sulfur oxidation, such as sor gene, need to be further verified via biochemical experiments, and it is critical to determine the features of key metabolic enzymes involved in sulfur oxidation mechanism, which warrants further investigations of this organism in the future.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

HY, XZ, XL, and YL designed the experiments, XZ and XL performed the experiments, HY, XZ and ZH analyzed the data, XG and QH carried out the annotation of draft genome sequence, YX, JC, LM and JN participated in molecular biologic experiments, XZ wrote the manuscript, XL, ZH and HY revised the manuscript. All authors read and approved the final manuscript.

Acknowledgments

The work was supported by the National Basic Research Program (973 Program, No. 2010CB630901).

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