Additional file 3: Figure S3.

Representative electromobility shift assays on 0.8% agarose gels. Effects of pH, NaCl and temperature on DNA–Imu3 complex relaxation. Lane 1: pUC19/EcoRI DNA (100 ng); lane 2: Imu3-pUC19/EcoRI untreated complex; lanes 3-7: Imu3-pUC19/EcoRI complex treated with pH values 10, 11, 12, 12.5, 13; lanes 8-11: Imu3-pUC19/EcoRI complex incubated 5 min at 100°C in the presence 0, 0.2, 0.5 and 1 M NaCl; lanes 12-14: complex treated with 0, 0.5 and 1 M NaCl at pH 12.

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Črnigoj et al. BMC Microbiology 2014 14:16   doi:10.1186/1471-2180-14-16