Figure 7.

Representative electromobility shift assays of re-ligated DNA previously complexed with Imu3 (0.8% agarose gels). Lane 1, 100 ng pUC19/EcoRI DNA; lane 2: 100 ng pUC19/EcoRI DNA purified with the QIAprep kit; lane 3: 100 ng pUC19/EcoRI DNA–Imu3 complex purified with the QIAprep kit; lane 4: ligation reaction of purified DNA; lane 5: ligation reaction of purified DNA–Imu3 complex; lane 6: restriction (EcoRI) of ligation reaction of purified DNA (from lane 4); lane 7: restriction (EcoRI) of ligation reaction of purified DNA–Imu3 complex (from lane 5). M: λ/PstI marker.

Črnigoj et al. BMC Microbiology 2014 14:16   doi:10.1186/1471-2180-14-16
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