Scheme of the adapted SHIME system (consisting of stomach, small intestine and ascending colon - AC - compartments) used for the long-term study. Two HMI modules have been connected in parallel to the vessel simulating the AC compartment in order to obtain information on bacterial adhesion and host response after 24 and 48 h. The SHIME system was fed three times per day with SHIME feed; the medium in the lower compartment of the HMI modules (containing Caco-2 cells) was fully replaced every 6 hours by means of an automatic pump. The exhausted medium was collected in order to analyze the concentration of IL-8.
Marzorati et al. BMC Microbiology 2014 14:133 doi:10.1186/1471-2180-14-133