Scheme of the HMI module for long-term studies of the host-microbiota interaction in the GIT. A polyamide semipermeable membrane and a mucus layer form a double functional layer that separates the luminal compartment (upper one) from the lower compartment containing enterocyte cell lines. The HMI module allows to study the bacterial adhesion under relevant shear forces and microaerophilic conditions. It allows the reciprocal exchange of signals and metabolites between compartments and it allows the exposure of cell lines to a complex microbial community, representative for the human colon, for up to 48 h.
Marzorati et al. BMC Microbiology 2014 14:133 doi:10.1186/1471-2180-14-133