PLC-expressing Mycobacterium tuberculosis more efficiently stimulates the cell activation, production of proinflammatory cytokines and NO2 in alveolar macrophages. Production of (A) the proinflammatory cytokines TNF-α, IL-1α, IL-1β, and IL6; (B) IL-10, determined by ELISA, and (C) NO, determined by Greiss reaction. (D) Quantification of phosphorylated p38, ERK1/2, JNK1/2, and PLC-γ determined by CBA (Cytometric Bead Array), and expressed as U/ mL. # P < 0.0001 for uninfected cells vs. infected cells (97-1505 or 97-1200); ***P < 0.0001; *P < 0.05 (one-way ANOVA). Data are representative of three (A–C) and two (D) independent experiments (error bars, s.e.m.).
Assis et al. BMC Microbiology 2014 14:128 doi:10.1186/1471-2180-14-128