Open Access Research article

Mycobacterium tuberculosis expressing phospholipase C subverts PGE2 synthesis and induces necrosis in alveolar macrophages

Patricia A Assis13, Milena S Espíndola13, Francisco WG Paula-Silva1, Wendy M Rios3, Priscilla AT Pereira1, Sylvia C Leão2, Célio L Silva3 and Lúcia H Faccioli1*

Author Affiliations

1 Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av Cafe, s/n, Ribeirão Preto, SP 14040-903, Brazil

2 Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil

3 Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil

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BMC Microbiology 2014, 14:128  doi:10.1186/1471-2180-14-128

Published: 19 May 2014

Additional files

Additional file 1: Figure S1:

Viabilidty of Mtb isolates after treatment with the PLC inhibitors D609 and U73122.

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Additional file 2: Figure S2:

Inhibition of Mycobacterial PLCs affects alveolar macrophage necrosis through the regulation of PGE2 synthesis.

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Additional file 3: Figure S3:

Resazurin metabolisation by Mtb isolates 97-1200 and 97-1505 and phagocytosis rate by alveolar macrophages.

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Additional file 4: Figure S4:

PLC activity assay.

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