Test for the heritability of persistence and elimination of persister cells. (A) Exponential grown S. suis strain 10 was treated with 100-fold MIC of gentamicin for three hours, and at indicated time points CFU were determined. Subsequently, surviving bacteria were incubated in fresh THB media overnight, then grown to early logarithmic phase and challenged with 100-fold MIC of gentamicin. This procedure was repeated for four consecutive cycles. The values are means of three biological replicates and error bars indicate the standard deviation. (B)S. suis strain 10 was sequentially grown to early exponential growth phase. At each cycle CFU of the initial inoculum and of surviving bacteria after a one-hour 100-fold MIC gentamicin challenge were determined. Data were expressed for each cycle as percentage of surviving bacteria in relation to the initial inoculum before antibiotic treatment. The dotted line represents the limit of detection. Standard deviation is shown for three replicates.
Willenborg et al. BMC Microbiology 2014 14:120 doi:10.1186/1471-2180-14-120