Interactions between chemically coupled, but physically separated populations. (A) Schematic of a microfabricated device of type-3, consisting of two parallel habitats (each of 85 patches) chemically coupled by 200 nm deep nanoslits of 15 × 15 μm, which allow for the diffusion of molecules but are too shallow for bacteria to pass through. (B) Area fraction occupied per patch (occupancy) for the top and bottom habitats, the top habitat is inoculated from the right and the bottom habitat from the left with the same initial culture of strain JEK1036 (green). (C) Kymograph where the fluorescence intensities of the top and bottom habitats are superimposed: cells in the top habitat are shown in red and cells in the bottom habitat in green. Note that both habitats are inoculated from the same (JEK1036) culture and that the bacteria in the upper and lower habitats are spatially confined to their own habitat.
van Vliet et al. BMC Microbiology 2014 14:116 doi:10.1186/1471-2180-14-116