Spore germination of slow-germinating strains and of gerAA disruption mutant complemented with gerA sequences from slow-germinating strains. ab: Germination of MW3∆gerAA (x), the wild-type strains ATCC14580 (■), NVH 1032 (▲), NVH1112 (●) and NVH800 (♦) measured as reduction in absorbance (A600) after addition of germinant (100 mM L-alanine). cd: Spore germination of the MW3∆gerAA (x), and MW3∆gerAA complemented with gerA from ATCC14580 (□ NVH1311), NVH1032 (∆ NVH1309), NVH1112 (○ NVH1321) and NVH800 (◊ NVH1322) measured as reduction in absorbance (A600) after addition of germinant (100 mM L-alanine). The results represent the average (SD) of three independent spore batches. The type strain derivate MW3 (dotted line) has been included in Figure 3D for comparison.
Madslien et al. BMC Microbiology 2014 14:101 doi:10.1186/1471-2180-14-101