Effect of low pH preincubation on S. Typhimurium sensitivity to MccJ25. The S. Typhimurium 14028s strain was incubated at 37°C during 0, 6 and 24 h in M9 medium pH 7 (grey bars) or pH 4.7 (black bars). At mentioned times, cells were washed, resuspended in PBS and then incubated for 6 h with or without MccJ25 (117.5 μM). Finally, the number of surviving bacteria (CFU mL-1) was determined by plating on LB agar. Values are presented as percentage of bacteria (CFU mL-1) obtained after MccJ25 treatment referred to the control (with no antibiotic addition). Error bars represent standard deviations from five independent experiments.
Pomares et al. BMC Microbiology 2013 13:95 doi:10.1186/1471-2180-13-95