Table 1

Bacterial strains and plasmids used in this study
Strain Description Reference
Acinetobacter sp.
Tol 5 Wild type strain [19]
G4 A Tol 5 mutant constructed by insertion of a FRT site in the upstream of ataA of Tol 5, Gmr, SacB This study
G4K1 A Tol 5 mutant constructed by additional insertion of a FRT site in the downstream of ataA of G4, Gmr, Kmr, SacB This study
4140 Unmarked ΔataA mutant of Tol 5 constructed by FLP/FRT recombination in G4K1 This study
E. coli
DH5α Host for routine cloning TaKaRa
S17-1 Donor strain for conjugation [4]
Plasmid
pJQ200sk Mobile plasmid, SacB, Gmr [32]
pK18mob Mobile plasmid, Kmr [33]
pLOI2224 Source of FRT sites, Kmr [34]
pFT-A Source of FLP recombinase and tetR, Ampr [34]
pJQFRT Gene replacement vector harboring a single FRT sequence, SacB, and Gmr This study
pKFRT Mobile plasmid harboring a single FRT sequence, Kmr This study
pKFRT/FLP Gene replacement vector harboring a single FRT sequence, FLP recombinase under the control of Ptet promoter, and Kmr This study
pJQFRT_AtaAupstream A 1.0-kb fragment containing the upstream region of ataA ligated into the BamHI site of pJQFRT This study
pKFRT/FLP_AtaAdownstream A 2.8-kb fragment containing the downstream region of ataA ligated into the BamHI site of pKFRT/FLP This study

Ishikawa and Hori

Ishikawa and Hori BMC Microbiology 2013 13:86   doi:10.1186/1471-2180-13-86

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