Table 1

Total animals (n = 4) demonstrating granuloma formations in liver tissues
K-MAP K-MAP + L-NP-51 L-MAP L-MAP + L-NP-51
Day 90 3/4 3/4 4/4 4/4
Day 135 2/2* 3/4 4/4 3/4
Day 180 3/4 2/4 2/4 3/4

Tissues were stained with Hemotoxylin & Eosin (H & E stain) prior to evaluation. For K-MAP samples at Day 135, only two sets of animal tissues were available for examination due to early expiration of animals before the harvest date (these data are highlighted with ‘*’). Control animals did not demonstrate granuloma formation at Day 90 and Day 180; Day 135 control animals were contaminated and were positive for granulomas in liver tissues. The data represent the number of animals that demonstrated granuloma formations per total animals examined (n =4). Experimental groups included are the following: animals fed normal chow and infected with viable MAP cells (Live-MAP; L-MAP); animals fed viable probiotics in chow and uninfected (Live NP-51; L-NP-51); animals fed viable probiotics in chow and infected with non-viable MAP cells (K-MAP + L-NP-51); animals fed viable probiotics in chow and infected with viable MAP cells (L-MAP + L-NP-51). These data demonstrate MAP infection of tissues regardless of viable or non-viable NP-51 consumption. Additionally, these data evidence that host tissues produce granulomas from exposure to K-MAP antigens.

Karunasena et al.

Karunasena et al. BMC Microbiology 2013 13:8   doi:10.1186/1471-2180-13-8

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