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Open Access Research article

Source tracking of human leptospirosis: serotyping and genotyping of Leptospira isolated from rodents in the epidemic area of Guizhou province, China

Shijun Li1*, Dingming Wang1, Cuicai Zhang2, Xiaoyu Wei1, Kecheng Tian1, Xiuwen Li2, Yixin Nie2, Ying Liu1, Guanghai Yao1, Jingzhu Zhou1, Guangpeng Tang1, Xiugao Jiang2 and Jie Yan34*

Author affiliations

1 Institute of Communicable Disease Control and Prevention, Guizhou Provincial Centre for Disease Control and Prevention, 73 Bageyan Road, Guiyang 550004, Guizhou, People's Republic of China

2 National Institute for Communicable Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, 155 Changbai Road, Changping District, 102206 Beijing, People's Republic of China

3 Department of Medical Microbiology and Parasitology, College of Medicine, Zhejiang University, Hangzhou, People's Republic of China

4 Laboratory of Microbiology and Parasitology, College of Medicine, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, Zhejiang, People's Republic of China

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Citation and License

BMC Microbiology 2013, 13:75  doi:10.1186/1471-2180-13-75

Published: 1 April 2013

Abstract

Background

Sustained human leptospirosis as well as death cases has been reported in Qiandongnan Prefecture, Southeast of Guizhou, China, recently, but these human patients were only clinically diagnosed, and leptospires have never been isolated from patients in these epidemic regions, In order to track the source of infection and understand the etiologic characteristic of leptospirosis, we performed rodent carrier surveillance for leptospirosis in the epidemic area in 2011. The population distribution of rodents in the epidemic regions was revealed.

Results

Four strains of leptospire were isolated from Apodemus agrarius. Microscopic agglutination test (MAT) confirmed the four isolates belonged to leptospiral serogroup Icterohaemorrhagiae. Multilocus sequence typing (MLST) indicated that all the four strains were defined as sequence type 1(ST1), which is identical to the three strains isolated from Rattus tanezumi in Rongjiang County in 2007. Clustering analysis of the MLST data indicated that the local isolates exactly matched with reference strain of leptospiral serovar Lai strain 56601, which is consistent with anti-Leptospira antibody detection of patients using MAT.

Conclusions

Apodemus agrarius may be the potentially important carrier of leptospirosis and the potential source of leptospiral infection in human, and serovar Lai maybe the epidemic serovar of Leptospira in the localities.

Keywords:
Rodents; Leptospira; Serotyping; Genotyping; Source of infection