Photomicrographs of longitudinal sections of small intestine of the experimental groups. Jejunum segments were collected and processed for optical microscopy analysis at the end of the experiment (day 58) (N = 8 mice per group). (NC), negative control group, figures A and D; (Bov) mice treated with bovicin HC5, figures B and E; (PC) positive control group, figures C and F. The sections were stained with hematoxylin and eosin (HE; left panel) or PAS/Alcian Blue (right panel). Abbreviations: L: lumen; EP: simple cuboidal epithelium; BB: brush border; V: villum; LP: lamina propria; LC: Lieberkühn crypt; Sm: submucosa; IC: inner circular muscle layer; OL: outer longitudinal muscle layer. The asterisks indicate intraepithelial lymphocytes; simple arrow indicates Paneth cells. Black arrow head indicates goblet cells PAS/AB+; red arrow head indicates PAS+ cells. Right panel – Scale bar: 100 μm; Left panel – Scale bar: 50 μm.
Paiva et al. BMC Microbiology 2013 13:69 doi:10.1186/1471-2180-13-69