Open Access Research article

Safety evaluation of the antimicrobial peptide bovicin HC5 orally administered to a murine model

Aline Dias Paiva1, Kenner Morais Fernandes2, Roberto Sousa Dias2, Alípio dos Santos Rocha2, Leandro Licursi de Oliveira2, Clóvis Andrade Neves2, Sérgio Oliveira de Paula2 and Hilário Cuquetto Mantovani1*

  • * Corresponding author: Hilário Cuquetto Mantovani

Author Affiliations

1 Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil

2 Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil

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BMC Microbiology 2013, 13:69  doi:10.1186/1471-2180-13-69

Published: 27 March 2013

Additional files

Additional file 1:

Cytokine production in spleen of five-week old female BALB/c mice treated with bovicin HC5 or ovalbumin. The relative expression of IL-12p40 (A), IFN-γ (B), IL-5 (C), IL-13 (D), TNF-α (E), TGF-β (F), IL-10 (G), IL-4 (H), IL-17 (I) mRNA was determined by real time-PCR and calculated by reference to the β-actin in each sample, using the threshold cycle (Ct) method. Results are shown as the mean value ± SD of duplicate samples from three independent mice within the NC, Bov and PC groups. Differences among treatments were indicated by different lowercase letters and were considered statistically significant by the Bonferroni multiple comparison test (p < 0.05). (NC) negative control group; (Bov) mice treated with bovicin HC5; (PC) positive control group. (TIFF 10328 kb)

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