The complex genetic context of mecA in WCH1. The context of mecA is displayed in two parts with the same lip gene shown in both parts. Numbers of orf are shown (e.g. 8 represents orf8), while IS431 is indicated as 431. PCR primers for mapping and linking are indicated. JCSC1435 is a S. haemolyticus strain. Several self-ligated restricted fragments that were used as templates for inverse PCR were indicated as fragments A to H with the restriction locations of the enzymes being shown. The restriction enzymes and primers for inverse PCR for each fragment are as below: A. HindIII, orf2_1-R1/ZZ-4; B. HaeIII, acf-R1/ZZ-3; C. NheI, orf24-1/ZZ-16; D. HhaI, feoB-F1/feoB-R1; E. EcoRI, ZZ-11/ZZ-12; F. HincII, ZZ-28/arsR-up1; G. HhaI, ZZ-28/ZZ-29; H. EcoRV, ZZ-30/ZZ-31. The 8-bp DR (CTTTTTGC) possibly generated by the insertion of Tn6191 is indicated. Black poles represent the IR of SCC. Genes with different origins are shown in different shading with those belonging to the mec complex in black and those of the core chromosome of S. haemolyticus in grey. Closest matches, if available, of certain regions are indicated. More information on genes for their closet matches and function is available in Table 1.
Zong BMC Microbiology 2013 13:64 doi:10.1186/1471-2180-13-64