Figure 6.

Expression of toll-like receptor negative regulators in bovine intestinal epithelial (BIE) cells. (A) BIE cells were stimulated with Lactobacillus casei OLL2768 or Pam3CSK4 for 12, 24, 36 or 48 hours and the expression of MKP-1, IRAK-M, SIGIRR, Bcl-3, Tollip and ABIN-3 negative regulators was studied. The results represent four independent experiments. Significantly different from control at the same time point *(P<0.05). (B) BIE cells were pre-treated with Lactobacillus casei OLL2768 or Pam3CSK4 for 48 hours and then stimulated with heat-stable Enterotoxigenic Escherichia coli (ETEC) pathogen-associated molecular patterns (PAMPs). The expression of MKP-1, IRAK-M, SIGIRR, Bcl-3, Tollip and ABIN-3 negative regulators was studied at the indicated times post-heat-stable ETEC PAMPs challenge. The results represent four independent experiments. Significantly different from ETEC control at the same time point *(P<0.05), **(P<0.01).

Takanashi et al. BMC Microbiology 2013 13:54   doi:10.1186/1471-2180-13-54
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