Open Access Research article

Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation

Naoya Takanashi1†, Yohsuke Tomosada1†, Julio Villena1,2†, Kozue Murata1, Takuya Takahashi1, Eriko Chiba1, Masanori Tohno1,3, Tomoyuki Shimazu1,4, Hisashi Aso5, Yoshihito Suda6, Shuji Ikegami7, Hiroyuki Itoh7, Yasushi Kawai1, Tadao Saito1, Susana Alvarez2 and Haruki Kitazawa1*

1 Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Aoba-ku, 981-8555, Japan

2 Laboratory of Clinical and Experimental Biochemistry, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina

3 National Agriculture and Food Research Organization, National Institute of Livestock and Grassland Science, Nasushiobara, 329-2793, Japan

4 Laboratory of Animal Breading and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555, Japan

5 Cell Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Aoba-ku, 981-8555, Japan

6 Department of Food, Agriculture and Environment, Miyagi University, Sendai, 982-0215, Japan

7 Division of Research and Development, Food Science Institute, Meiji Dairies Co, Kanagawa, Odawara, 250-0862, Japan

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BMC Microbiology 2013, 13:54 doi:10.1186/1471-2180-13-54

Published: 7 March 2013

Additional files

Additional file 1: Figure S1:

Selection of immunomodulatory lactobacilli. (A) BIE cells were pre-treated with different lactobacilli strains for 48 hours and the expression of MCP-1, IL-6 and IL-8 was studied. Values represent means and error bars indicate the standard deviations. The results represent five independent experiments. Significantly different from control *(P<0.05). (B) BIE cells were pre-treated with different lactobacilli strains for 48 hours and the stimulated with heat-stable ETEC PAMPs and then the expression of MCP-1, IL-6 and IL-8 was studied at hour twelve post-stimulation. Values represent means and error bars indicate the standard deviations. The results represent five independent experiments. Significantly different from ETEC control *(P<0.05).

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