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Open Access Research article

Integrin α5β1-fimbriae binding and actin rearrangement are essential for Porphyromonas gingivalis invasion of osteoblasts and subsequent activation of the JNK pathway

Wenjian Zhang1*, Jun Ju1, Todd Rigney2 and Gena Tribble2

Author Affiliations

1 Department of Diagnostic and Biomedical Sciences, University of Texas School of Dentistry at Houston, 7500 Cambridge Street, Suite 5366, Houston, TX, 77054, USA

2 Department of Periodontics and Dental Hygiene, University of Texas School of Dentistry at Houston, 7500 Cambridge Street, Houston, TX, 77054, USA

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BMC Microbiology 2013, 13:5  doi:10.1186/1471-2180-13-5

Published: 10 January 2013

Abstract

Background

Chronic periodontitis is an infectious disease of the periodontium, which includes the gingival epithelium, periodontal ligament and alveolar bone. The signature clinical feature of periodontitis is resorption of alveolar bone and subsequent tooth loss. The Gram-negative oral anaerobe, Porphyromonas gingivalis, is strongly associated with periodontitis, and it has been shown previously that P. gingivalis is capable of invading osteoblasts in a dose- and time-dependent manner resulting in inhibition of osteoblast differentiation and mineralization in vitro. It is not yet clear which receptors and cytoskeletal components mediate the invasive process, nor how the signaling pathways and viability of osteoblasts are affected by bacterial internalization. This study aimed to investigate these issues using an in vitro model system involving the inoculation of P. gingivalis ATCC 33277 into primary osteoblast cultures.

Results

It was found that binding between P. gingivalis fimbriae and integrin α5β1 on osteoblasts, and subsequent peripheral condensation of actin, are essential for entry of P. gingivalis into osteoblasts. The JNK pathway was activated in invaded osteoblasts, and apoptosis was induced by repeated infections.

Conclusions

These observations indicate that P. gingivalis manipulates osteoblast function to promote its initial intracellular persistence by prolonging the host cell life span prior to its intercellular dissemination via host cell lysis. The identification of molecules critical to the interaction between P. gingivalis and osteoblasts will facilitate the development of new therapeutic strategies for the prevention of periodontal bone loss.

Keywords:
Osteoblasts; Porphyromonas gingivalis; Integrins; Cytoskeleton; Signaling; Apoptosis