Figure 4.

Transcriptional activation of relBEF in protease- and polyphosphate kinase deficient strains. Cultures of BW25113 ∆lon, BW25113 ∆ppk, BW25113 ∆clpPXlon, and BW25113 ∆clpPXlonhslVU contained pVK11 (RelE), pSC3326 (MazF), pTX3 (MqsR), or pBAD-yafQ plasmid for toxin expression. Toxins were induced and RNA was extracted at timepoints −1 (before induction), 15 and 60 min; 10-μg aliquots were subjected to northern blotting and hybridized with probes relE (A) and relF (B). The full-length relBEF transcript is marked by arrowhead (◄).

Kasari et al. BMC Microbiology 2013 13:45   doi:10.1186/1471-2180-13-45
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