Differentiation of THP-1 cells by M. genitalium strains. A. Adherent THP-1 cells showing fluorescence. Images of adherent cells were acquired using confocal laser scanning microscope with 10X objective and 488 nm laser. G37, TIM207 and TIM262 are wild type, TIM207 mutant and TIM262 control M. genitalium strains, respectively. HKG37 represents heat killed bacteria of wild type M. genitalium. Flour, Fluorescence; DIC, Differential interference contrast. B. Graph showing the amount of adherent cells for each infection. The numbers of labeled cells in each image were counted using the particle plugin of Image J software. Average cell numbers from five different optical fields and from three independent experiments were used for determining the number of adherent mononuclear cells in each infection. * indicate significant difference from G37 (p≤0.01).
Martinez et al. BMC Microbiology 2013 13:44 doi:10.1186/1471-2180-13-44