Figure 1.

Production of recombinant His10MG207 protein and determination of phosphatase activity. A. Protein profiles of overexpressed and purified protein of His10MG207. Lanes: Marker, EZ-Run Rec Protein Ladder (Fisher Scientific); Un-induced, extracts of E. coli strain BL21 before the addition of 0.5 mM IPTG; Induced, extracts of E. coli strain BL21 after 3 h of the addition of 0.5 mM IPTG; His10MG207, purified His tagged MG207 protein after Ni-NTA affinity chromatography. Numbers on the left represent the sizes of the marker proteins in KDa. B. Phosphatase activity of His10MG207 with pNPP as substrate. Various amounts (μg) of purified His10MG207 protein (Protein) were added to the reaction mixture containing pNPP. Activity was measured in the presence of 5 mM MgCl2. Values represent Mean ± SD. C. Phosphatase activity of His10MG207 with synthetic threonine peptide as substrate. Various amounts (μg) of purified His10MG_207 protein (Protein) were added to the reaction mixture containing synthetic threonine phosphate (KRpTIRR). Values represent Mean ± SD.

Martinez et al. BMC Microbiology 2013 13:44   doi:10.1186/1471-2180-13-44
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