Open Access Highly Accessed Research article

A serine/threonine phosphatase encoded by MG_207 of Mycoplasma genitalium is critical for its virulence

Mario A Martinez12, Kishore Das1, Sankaralingam Saikolappan1, Luis A Materon2 and Subramanian Dhandayuthapani1*

Author Affiliations

1 Regional Academic Health Center and Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, Edinburg, TX, 78541, USA

2 Department of Biology, University of Texas Pan American, Edinburg, TX, 78539, USA

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BMC Microbiology 2013, 13:44  doi:10.1186/1471-2180-13-44

Published: 21 February 2013

Additional files

Additional file 1: Figure 1:

Viability of M. genitalium strains based on color change assay. M. genitalium G37, TIM207 and TIM262 were grown and harvested as described in method section. The bacteria were resuspended in appropriate amount of PBS to give an OD600 =1.0. Different volume of the inoculum, as indicated in the x-axis were added to 200 μl of SP-4 medium in a 96 well plate and incubated at 37°C for 6 h. Color change of SP-4 medium, due to the growth of mycoplasma, from red to orange was monitored by reading the plate at 620 nm in a microplate reader. Solid grey bars, dotted bars, solid black bars and horizontal stripped bars indicate absorbance (A620) of PBS, TIM207, G37 and TIM 262 respectively. The results indicate that there is no significant difference in viability between the strains at the time of harvest.

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Additional file 2: Table S1:

Mass spectrometry of analysis of 2D spots.

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Additional file 3: Figure S2:

Growth of M. genitalium G37 and TIM207 strains in the presence of glucose and glycerol. G37 and TIM207 was grown in a T-25 flask with SP-4 medium with either 1% (v/v) glucose or glycerol as carbon source until the color of the medium turns yellow (approximately 5 days, four different flasks for each strains). The bacteria were collected by scrapping and by centrifugation at 12,000 rpm for 15 min. The cells were washed two times in sterile PBS and finally suspended thoroughly with 23G syringe in 1 ml of sterile PBS and OD at 600 nm recorded. The solid bars and stripped bars indicate absorbance (A600) of either of strains grown in glucose and glycerol, respectively. “*” = p≤ 0.05 between TIM207 grown in glucose vs glycerol.

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