Figure 3.

L. jensenii induced NF-κB expression without immunogenic response. 24 h lysates and supernatants harvested from endocervical (End1/E6E7) epithelial cells cultured with 7x106L. jensenii 1153 wild type (WT), bioengineered L. jensenii 1153–1666, 2666, 3666 and 1646 strains or MALP-2 (50 nM) as a positive control. (Figure 3a) Luciferase activity measured in lysates from triplicate cultures in one representative of five experiments. Bars represent means and SEM ***P<0.001 different from medium control. (Figure 3b) IL-8 production analyzed in corresponding supernatants, bars are means and SEM from duplicate cultures in one representative of 11 experiments **P<0.01 different from medium control, ++P<0.01 different from L. jensenii WT. (Figure 3c) SLPI detected in the same supernatants, bars are mean and SEM of duplicate cultures in one representative of six experiments +P<0.05 different from L. jensenii WT.

Yamamoto et al. BMC Microbiology 2013 13:4   doi:10.1186/1471-2180-13-4
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