Resolution:
## Figure 3.
The hfq∆ mutant is deficient in anaerobic respiration. (A) Growth of MR-1/empty vector, MR-1/phfq, hfq∆ /empty vector, and hfq∆ /phfq under anaerobic conditions with fumarate as the terminal electron acceptor. Data
presented is from three independent cultures. Error bars represent a 99% confidence
interval (P = 0.01). (B and C) Results of chromium reduction assays. Chromium reduction/disappearance of Cr(VI)
was assayed using the diphenylcarbazide method. Error bars represent a 99% confidence
interval (P = 0.01). (D) Quantification of the rate of Cr(VI) reduction (expressed as the change in ABS_{541} per minute per ABS_{600}) in the cultures tracked in (C) above during the first five minutes following the addition of chromium to anaerobic
cultures. Error bars represent the standard deviation for triplicate cultures. **
indicates that the hfq∆ /empty vector rate is statistically different from the other three strains (P < 0.002
for all three comparison in unpaired two-tailed Student’s T-tests).
Brennan |