Shewanella oneidensis Hfq promotes exponential growth, terminal culture density, and viable cell counts in stationary phase. Growth of MR-1/empty vector, MR-1/phfq, hfq∆ /empty vector, and hfq∆ /phfq on LB agar containing kanamycin (A), in LB liquid containing kanamycin (B), or in modified M1 defined medium containing kanamycin (C). Plates in (A) were photographed after 24 hours of growth following inoculation from frozen permanent stocks. Three independent liquid cultures of each strain tracked in (B-D) were inoculated with log phase cultures grown in LB (B and D) or modified M1 medium (C). (D) Analysis of the relationship between viable cell counts (CFU/ml) and culture turbidity (ABS600) in LB cultures. Data points marked with “#” have CFU/ml/ABS600 values of zero. Error bars in panels (B-D) indicate a 99% confidence interval (P = 0.01).
Brennan et al. BMC Microbiology 2013 13:33 doi:10.1186/1471-2180-13-33